Figure 5: The predicted inactive members of the ß-lactamase fold family

a



YLR115w_Sc_2131782         DGSGTTVG---SVVRFDNVTLLIDPGWNPSKVSYEQCIKYWEKVIPE----IDVIILSQPTIECLGAHSLLYYNFTSHFISRIQVYATLPVINLGRVSTIDSYASAGVIG------PYDTNKLDLEDIEISFDHIVPLKYSQLVDLRSRYDGLTLLAYNAGVCPGGSIWCIST

CPSB_Bt_1706103            LSGVQEESALCYLLQVDEFRFLLDCGWDEH--FSMDIIDSLRKHVHQ----IDAVLLSHPDPLHLGALPYAVGKLG----LNCAIYATIPVYKMGQMFMYDLYQSRHNT--------EDFTLFTLDDVDAAFDKIQQLKFSQIVNLKGKGHGLSITPLPAGHMIGGTIWKIVK

F09G2.4_Ce_2435621         FSGAKDEGPLCYLLQVDGDYILLDCGWDER--FGLQYFEELKPFIPK----ISAVLISHPDPLHLGGLPYLVSKCG----LTAPVYATVPVYKMGQMFIYDMVYSHLDV--------EEFEHYTLDDVDTAFEKVEQVKYNQTVVLKGD-SGVHFTALPAGHMLGGSIWRICR

PF1_Sc_1077401             LGGSNEVGRSCHILQYKGKTVMLDAGIHPA-YQGLASLPFYDEFDLS---KVDILLISHFHLDHAASLPYVMQRTN----FQGRVFMTHPTKAIYRWLLRDFVRVTSIGSSSSSMGTKDEGLFSDEDLVDSFDKIETVDYHSTVDVNG----IKFTAFHAGHVLGAAMFQIEI

SPAC17G6.16c_Sp_2408029    LGAGNEVGRSCHVIQYKGKTVMLDAGVHPA-YTGLSALPFFDEFDLS---TVDVLLISHFHLDHVASLPYVMQKTN----FRGRVFMTHPTKAVCKWLLSDYVKVSNVG--------MEDQLYDEKDLLAAFDRIEAVDYHSTIEVEG----IKFTPYHAGHVLGACMYFVEM

CspA_Bt_1707412            LGAGQEVGRSCIILEFKGRKIMLDCGIHPG-LEGMDALPYIDLIDPA---EIDLLLISHFHLDHCGALPWFLQKTS----FKGRTFMTHATKAIYRWLLSDYVKVSNIS--------ADDMLYTETDLEESMDKIETINFHEVKEVAG----IKFWCYHAGHVLGAAMFMIEI

MTH1203_Mth_2622312        MGGFREVGRSCLYLQTPNSRVLLDCGVNVAGGDDKNSYPYLNVPEFT-LDSLDAVIITHAHLDHSGFLPYLYHYG-----YDGPVYCTAPTRDLMTLLQLDHIDIAHRE--------DEPLPFNVKHVKKSVKHTITLDYGEVTDIAPD---IRLTLHNAGHILGSAMAHLHI

AF0482_Af_2650146          LGGSREVGRSCYLLQTPESRILIDCGVNVS---NLSSTPYLYVPEVQPLDALDAVVITHAHLDHCGLVPLLYKFG-----YRGPIYLTPPTRDLMVLLQLDFLEVAGRE--------GTNPPYSSNLIREALKHTITLDYGVVTDISPD---VRLTFYNAGHILGSAIAHFHI



YLR115w_Sc_2131782         YS--EKLVYAKRWNHTRDNILNAASILDATGKPLSTLMRPSAIITTLDRF--GSSQP-FKKRSKIFKDTLKKGLSSDGSVIIPVDMSGKFLDLFTQVHELLFESTKINAHTQVPVLILSYARGRTLTYAKSMLEWLSPSLLKTWENRNNTSPFEIGSRIKIIAPNELSKY

CPSB_Bt_1706103            DGE-EEIVYAVDFNHKREIHLNGCSLEMLS--------RPSLLITDSFNA--TYVQPRRKQRDEQLLTNVLETLRGDGNVLIAVDTAGRVLELAQLLDQIWRTKDAGLG--VYSLALLNNVSYNVVEFSKSQVEWMSDKLMRCFED-KRNNPFQFRHLSLCHGLSDLARV

F09G2.4_Ce_2435621         VTG-EDIVYCVDFNHKKERHLNGCSFDNFN--------RPHLLITGAHHI--SLPQMRRKDRDEQLVTKILRTVRQKGDCMIVIDTAGRVLELAHLLDQLWSNADAGLS--TYNLVMMSHVASSVVQFAKSQLEWMNEKLFKYDSSSARYNPFTLKHVTLCHSHQELMRV

PF1_Sc_1077401             AG--LRVLFTGDYSREVDRHLNSAEVPPL---------SSNVLIVESTFG-TATHEP-RLNRERKLTQLIHSTVMRGGRVLLPVFALGRAQEIMLILDEYWSQHADELGGGQVPIFYASNLAKKCMSVFQTYVNMMNDDIRKKFRD-SQTNPFIFKNISYLRNLEDFQDF

SPAC17G6.16c_Sp_2408029    AG--VNILFTGDYSREEDRHLHVAEVPPK---------RPDVLITESTYG-TASHQP-RLEKEARLLNIIHSTIRNGGRVLMPVFALGRAQELLLILDEYWNNHLDLR---SVPIYYASSLARKCMAIFQTYVNMMNDNIRKIFAE-R--NPFIFRFVKSLRNLEKFDDI

CspA_Bt_1707412            AG--VKLLYTGDFSRQEDRHLMAAEIPNI---------KPDILIIESTYG-THIHEK-REEREARFCNTVHDIVNRGGRGLIPVFALGRAQELLLILDEYWQNHPELH---DIPIYYASSLAKKCMAVYQTYVNAMNDKIRKQINI-N--NPFVFKHISNLKSMDHFDDI

MTH1203_Mth_2622312        GDGQHNMVYTGDFKYEQSRLLEAAANRFP---------RIETLVMESTYGGHEDVQPSRNRAEKELVKTIYSTLRRGGKILIPVFAVGRAQELMIVLEEYIRTGIIDE----VP-VYIDGMIWEANAIHTARPEYLSKDLRDQIFH-MGHNPFISDIFHKVNGMDERREI

AF0482_Af_2650146          GEGHYNIAFTGDFKFEKTRLFDRAATNFP---------RLEALVMEATYGGPNDFQPSRKEAEERLIEVINRTLDRGGKVLIPTFAVGRSQEVMIVLEEAMREKRLRE----TY-VYLDGMIYEATAIHTAYPEYLNAQLRDLIFY-HGINPFISENFVRVDSSSKREEV





b



MG423_Mg_1351578      IKFFALGGQDERGKNCYVLEIDNDVFIFNVGSLTPTTAVLGVKKIIPDFSWIQENQARVKGIFIGNAITENLGSLEFLFHTVGFFPIYTSSIGASIIKSKINENKLNIARDKLEIHELKPLETIEISNH-SITPFKVSSSLPSSFGFALNTDNGYIVFIDDFIVLNDKNIAFE

YKQC_Bs_2833392       TAVFALGGLGEIGKNTYAVQFQDEIVLIDAGIKFPEDELLGIDYVIPDYTYLVKNEDKIKGLFITHGHEDHIGGIPYLLRQVNIP-VYGGKLAIGLLRNKLEEHGLLR-QTKLNIIGED--DIVKFRKT-AVSFFRTTHSIPDSYGIVVKTPPGNIVHTGDFKFDFTPV-GEP

SLR0551_Ssp_1723172   LKILPLGGLHEIGKNTCVFEYDDEILLLDAGLAFPTDDMHGVNVVLPDMTYLRENREKIKGMVVTHGHEDHIGGIAYHLKQFDIPIIYGPRLAMALLRDKLEEAGMLE-RTNLQTVSPR--EMVRLGKSFVVEFIRNTHSIADSYCLAIHTPLGVVMHSGDFKIDHTPIDGEF

HP14302_Hp_829454     VKITPLGGLGEIGGNMMVIETPKSAIVIDAGMSFPKEGLFGVDILIPDFSYLHQIKDKIAGIIITHAHEDHIGATPYLFKELQFP-LYGTPLSLGLIGSKFDEHGLKKYRSYFKIVEKR--CPISVGEF-IIEWIHITHSIIDSSALAIQTKAGTIIHTGDFKIDHTPVDNLP

MG139_Mg_1723112      TKIYAFGGIQEVGKNMYGIEYDDEIIIIDCGIKFASDDLLGINGIIPSFEHLIENQSKVKALFITHGHEDHIGGVPYLLKQVDIPVIYAPRIAASLILKKVNEHKDAK-LNKIVTFDDF--SEFQTKHF-KIDFYRVNHSIPDAFGICVQTPNGNIVQSGDYRFDFAAG-SEM



MG423_Mg_1351578      NQLNQIIPKLSDNTLLLITGVGLVGRNSGFTTPKHKSLEQLNRIITPAKGRIFVACYDSNAYSVMTLAQIARMQNRPFIIYSQSFVHLFNTIVRQKLFNNTHLNTISIEEINNS-TNSIVVLTSP-PDKLYAKLFKIGMNEDERIRYRKSDTFIFMTPKVAG-YEEIEAQILD

YKQC_Bs_2833392       ANLTKMAEIGKEGVLCLLSDSTNS-ENPEFTMSERRVGESIHDIFRKVDGRIIFATFASNIHRLQQVIEAAVQNGRKVAVFGRSMESAIEIGQTLGYINCPKNTFIEHNEINRMPANKVTILCTGSQGEPMAALSRIANGTHRQISINPGDTVVFSSSPIPGNTISVSRTINQ

SLR0551_Ssp_1723172   FDLQKVAEYGEKGVLCLLSDSTNA-EVPGITPSEASVIPNLDRVFSQAEGRLMVTTFASSVHRVNIILSLAQKHQRKVAVVGRSMLNVIAHARKLGYIKCPDNLFVPLKAARNLPDQQQLILTTGSQGEPLAAMTRISNGEHPQIKIRQGDTVVFSANPIPGNTIAVVNTIDR

HP14302_Hp_829454     TDLYRLAHYGEKGVMLLLSDSTNS-HKSGTTPSESTIAPAFDTLFKEAQGRVIMSTFSSNIHRVYQAIQYGIKYNRKIAVIGRSMEKNLDIARELGYIHLPYQSFIEANEVAKYPDNEILIVTTGSQGETMSALYRMATDEHRHISIKPNDLVIISAKAIPGNEASVSAVLNF

MG139_Mg_1723112      LDVHKVVKIAERNVHVFMSESTNA-EVPGFSQSEKLIYRNIQKILKEARGRVILTTFASNITRINEIIEIALNNKRKICLLGKSMDVNVNISRKIGLMAIDSNDIVEVRDIKNYPDRNILILCTGSQGEEAAALNTMARGKHNWVSLKSTDTIIMSSNPIPGNYAAVENLLNE



MG423_Mg_1351578      DIARNEVSYYN--LGREILSIQASDEDMKFLVSSLKPKYIIPTGGLYRDFINFTMVLKQAGAEQNQILILFNGEVLTIENKKLDSKKNELKLNPKCVDSAGLQEIGASIMFERDQMSESGVVIIIIYFDQKKSEFLNEITYSFLGVSLDVPEKDKLKT

YKQC_Bs_2833392       LYRAGAEVIHG-PLNDIHTSGHGGQEEQKLMLRLIKPKFFMPIHGEYRMQKMHVKLATDCGIPEENCFIMDNGEVLALKGDEASVAGKIPSGS-VYIDGSGIGDIGNIVLRDRRILSEEGLVIVVVSIDMDDFKISAGPDLISRGFVYMRESGD-LIN

SLR0551_Ssp_1723172   LMMQGANVIYG-KHQGIHVSGHASQEEHKMLLALTRPKFFVPVHGEHRMLVKHSQMAQAQGIPSENIVIVNNGDVIELTGDRIRVAGQVPSGI-ELVDQAGIVHESTMAERQQ--MAEDGLVTVAAALSKTG-TLLAYPEVHCRGVVMTIQPKL-LEE

HP14302_Hp_829454     LIKKEAKVAYQ-EFDNIHVSGHAAQEEQKLMLRLIKPKFFLPVHGEYNHVARHKQTAISCGVPEKNIYLMEDGDQVEVGPAFIKKVGTIKSGK-SYVDNQSNLSIDTSIVQQREEVASAGVFVATIFVNKNKQALLESSQFSSLGLVGFKDEKP-LIK

MG139_Mg_1723112      LSKFGVAIYENSSQLKLHASGHATQQELQLMLNLMFPKYLIPIHGEFKMMRTIKNIANECGIKSEDVALLSNGQVMYLIDEELYYSNEIINADPIYIESHNSSPDLARIIKQRQILSRDGMFAVIVVFDKNN-NIIGIPTLITRGCFFALDSNP-LMT





c



MAP_Rr_462569           CYIFPGGRGDSALFAVNG----FNILVDGGSDRKSCFWKLVRHLDR---IDSVLLTHIGADNLPGINGLLQRKVAELEEEQSQGSSSYSDWVKNLISPELGVVFFN-VPDKLRL-PDASRKAKRSIEEACLTLQHLNRLGIQAEP

ENP2_Tca_119358         CYIFPGGRGDSALFAVNG----FNILVDGGSERKSCFWKLVRHLDR---IDSILLTHIGADNLPGINGLLQRKIAEQDEEQSQGSTTYSDWMKNLISPELGVVFFN-VPDKLKT-QESSMRVKRSIEEACLTLQYLTKLGVNPEP

MJ0448_Mj_2495991       YKKFFAQHGFSALIEINN----KRILFDAGQN-SITLRENLRLFNEKEGFDYIVLSHGHYDHCDGLK-----YVIENDLING-KVIAHKD---AFLDKYAGNRYIG-IDEEIKE-YLLKKADLEIIEEPYKIDKDIIVSGYVPRE

PHAA011_PH_2696456      RKGLIGYHGFSALVEANG----YRVLVDTGTDGKVLLNNMSELGISPDDIDALFITHGHYDHTGGLR------ELLSARTEALDIYAHPGIFKRRLALKPKKREIG-IPFTREE-LEELGGKFILSDKSKEILPGFISSGEIERI

MJ0301_Mj_2495901       NSPFWSQHGLSFLIEVESNGIKKRILFDTATYAEPILFNMKLLNINPKSIDMIILSHNHFDHTGGLFG------IMKEINKEIPIFAHPNIFKVSFATEPEFMLAGTLNKTLKEDIEKLGGRWVLSRDPIRLMPGIFTLGEIEDE



MAP_Rr_462569           LYRVVSNTIEPLTLFHKMGVGRLDMYVLNPVKDSKEMQFLMQKWAG----NSKAKTGIVLANGKEAEIS---VPYLTSITALVVWLPANPTEKIVRVLFPGNAPQNKILEGLEKLRHLD-FLRYPVATQKDLAAGAVPANLKPS

ENP2_Tca_119358         LNRVVTATIDPIPLFHKMGVGRLDMYILNPVKDSKEMQFLMQKWAG----NSKAKTGIKLPNGKEGEIS---VPYLTSITALVVWHPANPSEKIVRVLFPGNAPQNKILEGLEKLKHLD-FLKYPVPTHKDIAGPLTPPVVKQT

MJ0448_Mj_2495991       YEY-------EMEEFQCIKDG---KRVKDEVNDDM---FLIAKGIL---ITGCSHSGIINVVEYGKKLS---EIKGVLGGFHLVGVSDNYLNRIVDYF--------KSQDFWIMPMHCTGFKALTKLSQ--LNNFVYGHVGKII

PHAA011_PH_2696456      TWD---------RAVGYFPDG-----SKDPVRDDMA--LIVEEGSKSVVITGCGHSGIINIAIHSKKLTNN-RIAALIGGFHLKGIERSLLDEAVKKL-------RELEIEKLYPGHCTGIEEFAYLWS-KFGNVEGIHVGKEI

MJ0301_Mj_2495901       EKINFEK--KPTIGLYKLENG---RVVLDNVEDEIGLAIVTEKGLI--IVSGCSHPGIVSMVKKSIKISGINKVYAVIGGFHLIDADNERIVSTIKAL-------KKLGVKKICTGHCTGFKAENMFMEEFKEDFERLHAGKII