Table 1: Validation based on comparison of annotated kinases in A. nidulans with their S. cerevisiae homolog

Annotated A. nidulans kinases	Function	S. cerevisiae homolog	Function	E-value
HogA	MAP kinase, homologous to the S. cerevisiae HOG1 gene, regulated by salt stress [17]	Hog1p	Osmoregulation, Activated under stress conditions when the cAMP cellular content is low [32]	10-155
UvsB	Control cell cycle checkpoint responses to DNA damage and incomplete replication [36]	Mec1p	Required for mitotic growth, DNA repair and meiotic recombination [37]	10-145
PkaC	Involved in G Protein Signaling Pathway regulating Morphological & Chemical Transitions [38]	Pka3p	Involved in G protein signaling pathway regulating pseudohyphal growth [32]	10-143
MpkC	Ser/Thr protein kinase family ATP binding, MAPK and transferase activity [34]	Hog1p	Osmoregulation, Activated under stress conditions when the cellular cAMP is low [32]	10-132
MpkB	Ser/Thr protein kinase family ATP binding, MAPK and transferase activity [34]	Fus3p	Regulates the mating and filamentation pathways, Ser/Thr protein kinase family ATP binding, MAPK and transferase activity [34]	10-120
Cdc2	Control of the eukaryotic cell cycle, required in higher cells for entry into S-phase and mitosis [34]. Required for continued nuclear DNA replication [39]	Cdc28p	Essential for the completion of the start, the controlling event, in the cell cycle [34]. Involved in G1/S transition, G2/M transition and S-phase of mitosis and in regulation of meiosis [32]	10-118
PhoA(M1)	Developmental response to phosphorus-limited growth, High sequence homology with Pho85p [33], Ser/Thr protein kinase [34]	Pho85p	Involved in phosphate and glycogen metabolism and cell cycle progression [32], Ser/Thr protein kinase [34]	10-111
PhoA(M47)	Developmental response to phosphorus-limited growth High sequence homology with Pho85p [33], Ser/Thr protein kinase [34]	Pho85p	Involved in phosphate and glycogen metabolism and cell cycle progression [32], Ser/Thr protein kinase [34]	10-111
Kcc1	Ser/Thr protein kinase family, CaMK subfamily [34]	Cmk2p	Important in cellular regulation, Ser/Thr protein kinase family, CaMK subfamily [34]	10-99
SldA	Important role in mitosis, homologous to Bub1p [40]	Bub1p	Mitotic Spindle Checkpoint gene [41]	10-90
KapR	Regulator of cAMP-dependent protein kinase PKAC catalytic subunit [34]	Sra1p	cAMP-dependent protein kinase regulatory subunit involved in glycogen accumulation, heat shock resistance & sporulation [32]	10-72
MpkA	Involved in polarized growth [42], Ser/Thr protein kinase family [34]	Slt2p	Involved in polarized cell growth [43], Ser/Thr protein kinase family [34]	10-71
NimA	Important role in mitotic regulation, required for spindle formation & nuclear envelope breakdown [34]	Kin3p	Involved in chromosome segregation [34]. Similar to NimA [31]	10-69
CmkB	Required for Normal Nuclear Division [44], Ser/Thr protein kinase family [34]	Cmk1p	Important in cellular regulation, Ser/Thr protein kinase family, CaMK subfamily [34]	10-62
AnkA	Encodes a Tyrosine kinase and Inhibitory phosphorylation on Tyr-15 of NIMXcdk1. Ortholog of Wee1p/Swe1p [45]	Swe1p	Encodes a Tyrosine kinase and Inhibitory phosphorylation on Tyr-15 of the main cell-cycle cyclin-dependent kinase Cdc28p [46]	10-61
SepH	Required for construction of the actin ring during cytokinesis [47]	Cdc15p	Essential for late nuclear division in the mitotic cycle. Required for inactivation of CDC2 kinase activity at the end of mitosis [32, 34]. Involved in cytokinesis [32].	10-50
CmkC	Required for Normal Nuclear Division [44], Ser/Thr protein kinase [34]	Ygl179cp	Ser/Thr protein kinase [32]	10-44
PtkA	Ser/Thr protein kinase family ATP binding, MAP Kinase and transferase activity, Regulation of DNA-dependent transcription [34]	Sgv1p	Required for recovery from pheromone-induced growth arrest in the cell cycle G1 phase, vegetative growth, Ser/Thr protein kinase family [34]	10-39