Table 9: S. cerevisiae autophagy proteins and their A. nidulans homologs
Group S. cerevisiae protein A. nidulans protein E-value
Cargo selection and tethering Vps45 Cytosolic and peripheral membrane protein AN6531.2 hypothetical protein similar to VpsB 10-111
Vps52 Involved in localization of actin and chitin AN4014.2 hypothetical protein 10-54
Vps53 Hydrophilic protein peripherally associated with the late Golgi AN2736.2 hypothetical protein 10-52
Vps54 Involved in vacuole biogenesis AN7993.2 hypothetical protein 10-40
Tlg2 tSNARE that affects a Late Golgi compartment AN2048.2 hypothetical protein 10-36
Cvt9 Involved in cytoplasm-to-vacuole targeting pathway and pexophagy AN2887.2 hypothetical protein 10-12
Cvt19 Involved in the cytoplasm-to-vacuole targeting pathway and in autophagy No homolog found
Vps51 Whiskey (whi) mutant; forms a tetramer with VPS52, VPS53, and VPS54 No homolog found
VFC - PtdIns 3-kinase complex Vps34 Phosphatidylinositol 3-kinase AN4709.2 hypothetical protein 10-167
Vps15 Myristoylated Serine/threonine protein kinase involved in vacuolar protein sorting AN0576.2 hypothetical protein 10-144
Apg6 Involved in vacuolar protein targeting No homolog found
Apg14 Involved in autophagy No homolog found
VFC - Conjugation systems Apg7 Similar to ubiquitin-activating enzymes, involved in autophagy AN7428.2 hypothetical protein 10-95
Aut2 Anchor protein, mediates attachment of autophagosomes to microtubules AN3470.2 hypothetical protein 10-60
Aut1 Involved in autophagocytosis AN7718.2 hypothetical protein 10-51
Aut7 Aut7p has homology to LC3, a microtubule-associated protein from rat AN5131.2 hypothetical protein similar to IDI-7 10-50
Apg12 Involved in autophagy AN1760.2 hypothetical protein 10-14
Apg5 Involved in autophagy AN5174.2 hypothetical protein 10-12
Apg16 Involved in autophagy AN0090.2 hypothetical protein 10-6
Apg10 Involved in autophagy; protein-conjugating enzyme involved in the Apg12p-Apg5p conjugation pathway No homolog found
VFC - Regulatory machinery Apg1 Protein kinase AN1632.2 hypothetical protein 10-109
Cvt18 Required for cytoplasm to vacuole targeting of proaminopeptidase I and starvation induced autophagy (AUT10). Needed for pre-Meiotic Replication (NMR1) AN0127.2 hypothetical protein 10-77
Cvt13 Sorting NeXin AN3584.2 hypothetical protein 10-72
Cvt20 Involved in transport of aminopeptidase I (Lap4p) through the cytoplasm-to-vacuole targeting pathway AN6351.2 hypothetical protein 10-48
Vac8 An armadillo repeat-containing protein localized on the vacuolar membrane AN2142.2 hypothetical protein similar to AF465210_1 karyopherin alpha 10-16
Apg17 Required for activation of Apg1 protein kinase AN6360.2 hypothetical protein 10-10
Apg13 Involved in autophagy AN2076.2 hypothetical protein 10-6
VFC - Apg9 complex Apg9 Integral membrane protein AN3734.2 hypothetical protein 10-110
Apg2 Required for sporulation AN5491.2 hypothetical protein 10-86
VFC - Early Sec pathway Sec23 Cytoplasmic GTPase-activating protein AN0261.2 conserved hypothetical protein 0
Sec24 Vesicle coat component AN3720.2 hypothetical protein 0
Sec16 Vesicle coat component AN6615.2 hypothetical protein 10-22
Sec12 Guanine nucleotide exchange factor for Sar1p No homolog found
Docking and fusion Vps11 Peripheral vaculor membrane protein; putative Zn-finger protein AN2461.2 hypothetical protein 10-82
Vps18 Vacuolar membrane protein AN2266.2 hypothetical protein similar to DigA protein 10-72
Vps16 Vacuolar sorting protein AN6911.2 hypothetical protein 10-53
Vps33 Involved in vacuolar protein targeting AN2418.2 hypothetical protein similar to vacuolar sorting protein 10-36
Vps41 Component of vacuolar membrane protein complex AN4876.2 hypothetical protein 10-34
Mon1 Unknown AN2093.2 hypothetical protein 10-34
Vps39 Involved in vacuolar morphogenesis AN6720.2 hypothetical protein 10-31
Vti1 v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p AN1973.2 hypothetical protein 10-22
Vam7 Hydrophilic protein, heptad repeat motif AN4551.2 hypothetical protein 10-14
Vam3 Member of the syntaxin family of proteins; predicted C-terminal TMD AN4416.2 hypothetical protein 10-13
Sec18 Cytoplasmic protein involved in protein transport between ER and Golgi; ATPase AN3098.2 hypothetical protein 10-171
Ypt7 Gtp-binding protein of the rab family; required for homotypic fusion event in vacuole inheritance, for endosome-endosome fusion, and for fusion of endosomes to vacuoles when expressed from high copy plasmid AN0089.2 RAB7_NEUCR Probable Ras-related protein Rab7 10-72
Sec17 peripheral membrane protein required for vesicular transport between ER and Golgi AN6267.2 hypothetical protein 10-51
Ccz1 Involved in sporulation, caffeine, calcium, and zinc sensitivity No homolog found
Vesicle breakdown Pep4 Vacuolar proteinase A AN2903.2 hypothetical protein similar to pepsinogen 10-145
Prb1 Vacuolar protease B AN0238.2 hypothetical protein 10-123
Aut4 Membrane transporter AN7437.2 hypothetical protein 10-65
Cvt17 Lipase, required for intravacuolar lysis of autophagic bodies AN5919.2 hypothetical protein 10-39
VFC - Vesicle Formation/Completion