- Communication -
| In Silico Biology 1, 0011 (1998); ©1998, Bioinformation Systems e.V. |
In silico cloning of a new protein kinase, Aik2, related to Drosophila aurora using the new tool: EST Blast
Claude Prigent1, Rob Gill2, Mike Trower3 and Philippe Sanseau3*
1 Departement de Biologie et Genetique du Developpement, CNRS UPR41, Universite de Rennes I, 35042, Rennes, France
2 Advanced Technology and Informatics Unit, Gunnels Wood Road, Stevenage, SG1 2NY, U.K.
3 Glaxo-Wellcome, Genome Informatics Group, Genomics Unit
* corresponding author
E-mail: ps14446@glaxowellcome.co.uk
Edited by T. Gaasterland; received April 23, 1998; accepted August 24, 1998
Abstract
Expressed Sequence Tags (ESTs) are short sequences derived from one or both ends of cDNA clones [Wilcox et al., 1991; Adams et al., 1992] that have been obtained from a variety of organisms. EST sequences are deposited in a database, dbEST [Boguski et al., 1993], which contained 1,539,270 sequences as of 27 March 1998. Since cDNA clones derived from the same transcript could be sequenced more than once and the information can start at different positions in the transcript it is possible to rebuild in silico a gene. With the growing number of sequences in dbEST in silico cloning of transcripts of interest and new members of gene families is rapidly becoming a method of choice when little experimental data is available. Recently we have developed a Web-based tool, EST Blast [Gill et al., 1997] that allow us to perform rapid and dynamic in silico assembly of ESTs.
In this short communication we report for the first time to our knowledge the use of ESTBlast to in silico clone a new gene and a step by step description of this particular in silico cloning project.