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In Silico Biology 7, 0001 (2006); ©2006, Bioinformation Systems e.V.  



Prediction of 3-dimensional structure of salivary odorant-binding protein-2 of the mosquito Culex quinquefasciatus, the vector of human lymphatic filariasis

Rajaiah Paramasivan*, Ramamoorthy Sivaperumal1, Kutty Jegadeeswaran Dhananjeyan, Velayutham Thenmozhi and Brij Kishore Tyagi

Centre for Research in Medical Entomology (Indian Council of Medical Research), 4, Sarojini Street, Chinna Chokkikulam, Madurai-625002, Tamil Nadu, India

1 Department of Bioinformatics, Bharathiar University, Coimbatore, Tamil Nadu, India.



* Corresponding author
   Email: rpsivan2000@yahoo.co.in


Edited by H. Michael; received April 21, 2006; revised November 15, 2006; accepted November 16, 2006; published January 08, 2007


Abstract

Olfaction of insects is currently recognized as the major area of research for developing novel control strategies to prevent mosquito-borne infections. A 3-dimensional model (3D) was developed for the salivary gland odorant-binding protein-2 of the mosquito Culex quinquefasciatus, a major vector of human lymphatic filariasis. A homology modeling method was used for the prediction of the structure. For the modeling, two template proteins were obtained by mGenTHERADER, namely the high-resolution X-ray crystallography structure of a pheromone-binding protein (ASP1) of Apis mellifera L., [1R5R:A] and the aristolochene synthase from Penicillium roqueforti [1DI1:B]. By comparing the template protein a rough model was constructed for the target protein using MODELLER, a program for comparative modelling. The structure of OBP of the mosquito Culex quinquefasciatus resembles the structure of pheromone-binding protein ASP1 of Apis mellifera L., [1R5R:A]. From Ramachandran plot analysis it was found that the portion of residues falling into the most favoured regions was 86.0%. The predicted 3-D model may be further used in characterizing the protein in wet laboratory.


Keywords: salivary odorant-binding protein-2, homology modeling, pheromone-binding protein, aristolochene synthase, Culex quinquefasciatus, 3-D model, MODELLER, mGenTHERADER, X-ray crystallography, predictive model, Swiss-PdbViewer, PROCHECK