- Article -
| In Silico Biology 7, 0019 (2007); ©2007, Bioinformation Systems e.V. |
ISN1 nucleotidases and HAD superfamily protein fold: in silico sequence and structure analysis
Bharath Srinivasan and Hemalatha Balaram*
Molecular Biology and Genetics Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Jakkur, Bangalore 560 064, India
* Corresponding author
Email: hb@jncasr.ac.in
Edited by E. Wingender; received November 11, 2006; revised February 16, 2007; accepted February 24, 2007; published February 28, 2007
Abstract
cN-II class of 5' purine nucleotidases exhibit specificity for IMP/GMP and belong to the HAD (haloacid dehalogenase) superfamily of hydrolases. The recently identified ISN1 class of IMP specific 5'-nucleotidases occurring in yeast, fungi and certain Plasmodia lack sequence homology with the cN-II class of enzymes. We show from analysis of motif and fold conservation that ISN1s also belong to the HAD superfamily. This identification adds a new novel member to this superfamily.
Keywords: purine nucleotidase, ISN1 nucleotidase, HAD superfamily, distant homology modeling, phylogenetic analysis